Wang H, Nuttall P A
NERC Institute of Virology and Environmental Microbiology, Oxford.
Parasitology. 1995 Aug;111 ( Pt 2):161-5. doi: 10.1017/s0031182000064908.
A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs, as determined by SDS-polyacrylamide gel electrophoresis, differed between species. In R. appendiculatus 2 IGBP bands were detected in haemolymph collected from unfed females and males, and an additional 78 kDa IGBP was detected in haemolymph samples of male and female ticks that had fed for 6 days. Although there was no difference in the IGBP profile of SGE derived from unfed male and female R. appendiculatus, 2 new IGBPs were detected in the SGE of the male ticks after they had fed for 6 days. The possible function of IGBP in ticks is discussed.
使用豚鼠免疫球蛋白G连接的琼脂糖柱来检测硬蜱（微小牛蜱、变异革蜱和六角硬蜱）中的免疫球蛋白G结合蛋白（IGBP）。在这3种蜱未进食个体的唾液腺提取物（SGE）中均发现了IGBP。通过SDS聚丙烯酰胺凝胶电泳测定，不同种类蜱中IGBP的大小有所不同。在未进食的微小牛蜱雌、雄个体采集的血淋巴中检测到2条IGBP条带，在已进食6天的雌、雄蜱血淋巴样本中还检测到一条额外的78 kDa的IGBP。尽管未进食的微小牛蜱雌、雄个体SGE的IGBP图谱没有差异，但雄蜱进食6天后，在其SGE中检测到2种新的IGBP。文中讨论了IGBP在蜱中的可能功能。
