一种来自西非豆（豆科）的具有杀虫活性的N-乙酰葡糖胺特异性凝集素基因。
An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae).
作者信息
Zhu K, Huesing J E, Shade R E, Bressan R A, Hasegawa P M, Murdock L L
机构信息
Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Plant Physiol. 1996 Jan;110(1):195-202. doi: 10.1104/pp.110.1.195.
Abstract
Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-binding and insecticidal activity against cowpea weevil, indicating that glycosylation and multimeric structure are not required for these properties. These results support the hypothesis that genes of the legume lectin gene family encode proteins that function in plant defense against herbivores.
摘要
西非单叶豆凝集素II（Griffonia simplicifolia II）是一种对N-乙酰葡糖胺具有特异性的豆科植物凝集素，当喂食给豇豆象（Callosobruchus maculatus (F.)）时具有杀虫活性。从叶片cDNA文库中分离出编码西非单叶豆凝集素II的cDNA克隆，进行测序并在细菌表达系统中表达。重组蛋白表现出对N-乙酰葡糖胺的结合能力以及对豇豆象的杀虫活性，这表明这些特性不需要糖基化和多聚体结构。这些结果支持了豆科凝集素基因家族的基因编码在植物抵御食草动物中起作用的蛋白质这一假说。
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