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豌豆中一种营养性凝集素的预测序列和结构。

Predicted sequence and structure of a vegetative lectin in Pisum sativum.

作者信息

Pak J H, Hendrickson T, Dobres M S

机构信息

Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.

出版信息

Plant Mol Biol. 1992 Mar;18(5):857-63. doi: 10.1007/BF00019200.

Abstract

We report the predicted sequence of four vegetative homologues (Blec1,2,3 and 4) of the pea seed lectin. This study indicates that, in contrast to the single-copy pea seed lectin (Kaminski et al., Plant Mol Biol 9:497-507, 1987), the pea vegetative lectin is transcribed by at least four members of a highly conserved multigene family whose members are only distantly related to the pea seed lectin at the primary amino-acid sequence level. For example, Blec1 shares only 38% amino-acid identity with the pea seed lectin. However, molecular homology modelling predicts that Blec1 probably forms a similar tertiary structure to the pea seed lectin.

摘要

我们报道了豌豆种子凝集素的四种营养同源物(Blec1、2、3和4)的预测序列。这项研究表明,与单拷贝的豌豆种子凝集素(卡明斯基等人,《植物分子生物学》9:497 - 507,1987年)不同,豌豆营养凝集素由一个高度保守的多基因家族的至少四个成员转录而来,这些成员在一级氨基酸序列水平上与豌豆种子凝集素仅有远缘关系。例如,Blec1与豌豆种子凝集素仅具有38%的氨基酸同一性。然而,分子同源性建模预测Blec1可能形成与豌豆种子凝集素相似的三级结构。

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