Wamwayi H M, Fleming M, Barrett T
National Veterinary Research Centre, Muguga, Kikuyu, Kenya.
Vet Microbiol. 1995 May;44(2-4):151-63. doi: 10.1016/0378-1135(95)00008-x.
Isolates of rinderpest virus (RPV) recovered from outbreaks of the disease in Kenya and Southern Sudan between 1986 and 1993 were compared to each other and to earlier isolates from East and West Africa. The recent isolates were mildly pathogenic for susceptible cattle and thus resembled other mild strains of RPV recovered from cattle and wildlife in East Africa more than 30 years ago. Monoclonal antibody analysis using a panel of 12 anti-RPV haemagglutinin protein-specific antibodies (mAbs) revealed that individual isolates were distinguishable. However, the panel of mAbs could not be used to relate the isolates on the basis of their pathogenicity or geographic origin. Immunoprecipitation of the virus-induced proteins from infected Vero cells, followed by SDS-polyacrylamide gel electrophoresis, showed that the recent mild RPV isolates from eastern Africa were closely related to each other and to their contemporary isolates from Nigeria and Egypt, but they were distinct from another mild isolate recovered from the region three decades ago. Two distinct lineages of African RPV isolates were identified by sequencing a region of the genome around the proteolytic enzyme cleavage site of the fusion protein from the old and new isolates. One lineage, which included virus isolates recovered from East and West Africa during the 1960s, showed a closer phylogenetic relationship to Asian and Middle Eastern RPV isolates. The other lineage consisted mainly of isolates recovered from East, West and North Africa between 1983 and 1993. The results showed that there was co-circulation of two different lineages of RPV in Nigeria during the epizootics of the 1980s.
对1986年至1993年间从肯尼亚和苏丹南部疾病暴发中分离出的牛瘟病毒（RPV）毒株，相互之间以及与早期从东非和西非分离出的毒株进行了比较。最近分离出的毒株对易感牛的致病性较弱，因此与30多年前从东非的牛和野生动物中分离出的其他温和型RPV毒株更为相似。使用一组12种抗RPV血凝素蛋白特异性单克隆抗体（mAbs）进行的单克隆抗体分析表明，各个分离株是可区分的。然而，这组mAbs无法根据其致病性或地理来源将这些分离株联系起来。对感染的Vero细胞中病毒诱导的蛋白质进行免疫沉淀，随后进行SDS-聚丙烯酰胺凝胶电泳，结果表明，来自东非的最近的温和型RPV分离株彼此之间以及与来自尼日利亚和埃及的同时期分离株密切相关，但它们与30年前从该地区分离出的另一种温和型分离株不同。通过对新旧分离株融合蛋白的蛋白水解酶切割位点周围的基因组区域进行测序，鉴定出了非洲RPV分离株的两个不同谱系。一个谱系包括20世纪60年代从东非和西非分离出的病毒株，与亚洲和中东的RPV分离株显示出更密切的系统发育关系。另一个谱系主要由1983年至1993年间从东非、西非和北非分离出的毒株组成。结果表明，在20世纪80年代的动物疫情期间，尼日利亚存在两种不同谱系的RPV共同传播。
