The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK.
Biomathematics and Statistics Scotland, JCMB, The King's Buildings, Peter Guthrie Tait Road, Edinburgh, EH9 3FD, Scotland, UK.
Sci Rep. 2020 Apr 16;10(1):6563. doi: 10.1038/s41598-020-63707-z.
When rinderpest virus (RPV) was declared eradicated in 2011, the only remaining samples of this once much-feared livestock virus were those held in various laboratories. In order to allow the destruction of our institute's stocks of RPV while maintaining the ability to recover the various viruses if ever required, we have determined the full genome sequence of all our distinct samples of RPV, including 51 wild type viruses and examples of three different types of vaccine strain. Examination of the sequences of these virus isolates has shown that the African isolates form a single disparate clade, rather than two separate clades, which is more in accord with the known history of the virus in Africa. We have also identified two groups of goat-passaged viruses which have acquired an extra 6 bases in the long untranslated region between the M and F protein coding sequences, and shown that, for more than half the genomes sequenced, translation of the F protein requires translational frameshift or non-standard translation initiation. Curiously, the clade containing the lapinised vaccine viruses that were developed originally in Korea appears to be more similar to the known African viruses than to any other Asian viruses.
当牛瘟病毒（RPV）于 2011 年宣布被根除时，这种曾经令牲畜闻风丧胆的病毒在世界上仅存的样本就只有保存在各个实验室中的那些了。为了在销毁本研究所持有的 RPV 库存的同时，仍然能够在需要时恢复各种病毒，我们已经确定了所有 RPV 不同样本的全基因组序列，包括 51 株野生型病毒和三种不同类型的疫苗株。对这些病毒分离物序列的研究表明，非洲分离株形成了一个单一的、不同的分支，而不是两个独立的分支，这与该病毒在非洲的已知历史更为一致。我们还发现了两组在 M 和 F 蛋白编码序列之间的长非翻译区中额外获得 6 个碱基的山羊传播病毒，并且表明，对于超过一半测序的基因组，F 蛋白的翻译需要翻译移码或非标准翻译起始。奇怪的是，最初在韩国开发的兔化疫苗病毒所在的分支似乎与已知的非洲病毒更为相似，而与任何其他亚洲病毒都不相似。
