Young C R, Eckersall P D, Saini P K, Stanker L H
US Department of Agriculture, Agricultural Research Service, Food Animal Protection Research Laboratory, College Station, TX 77845, USA.
Vet Immunol Immunopathol. 1995 Nov;49(1-2):1-13. doi: 10.1016/0165-2427(95)05454-e.
Haptoglobin (Hp) is an acute phase protein. The plasma concentration of Hp increases rapidly following tissue damage associated with infection and inflammation. Thus Hp levels could be used as a screening test for organic disease, an objective index of disease activity and response to therapy, or as a sign of microbial infection. Recently, an enzyme-linked immunosorbent assay for bovine Hp was described. We have now developed three different immunoassay formats for bovine Hp and report on their validation and relative value to the diagnosis of bovine disease. Hp levels measured using these three immunoassays were compared and contrasted with results obtained for Hp estimation as measured by the increase in the protection of peroxidase activity against acid inactivation following binding with bovine haemoglobin. The quantitative Hp immunoassays evaluated in the present study are simple, rapid, inexpensive, reproducible, and well suited for both field and laboratory use.
触珠蛋白（Hp）是一种急性期蛋白。在与感染和炎症相关的组织损伤后，血浆中Hp的浓度会迅速升高。因此，Hp水平可作为器质性疾病的筛查试验、疾病活动和治疗反应的客观指标，或作为微生物感染的标志。最近，有人描述了一种用于检测牛Hp的酶联免疫吸附测定法。我们现在已经开发出三种不同的免疫测定形式用于检测牛Hp，并报告了它们的验证情况以及在牛病诊断中的相对价值。将使用这三种免疫测定法测得的Hp水平与通过与牛血红蛋白结合后过氧化物酶活性对酸灭活的保护作用增加来测定Hp时获得的结果进行了比较和对比。本研究中评估的定量Hp免疫测定法简单、快速、廉价、可重复，非常适合现场和实验室使用。
