Otto C M, Rawlings C A
University of Pennsylvania, Department of Clinical Studies-Philadelphia 19104-6010, USA.
Vet Immunol Immunopathol. 1995 Nov;49(1-2):183-8. doi: 10.1016/0165-2427(95)05456-g.
Supernatants from feline peritoneal exudate cells (PECs) exposed to lipopolysaccharde (LPS) produced significantly (P < 0.05) more tumor necrosis factor (TNF) activity than supernatants from cells exposed to media. An in vitro LPS response was obtained following incubation of whole blood with 10 micrograms ml-1 LPS for 2 h. Intravenous infusion of LPS (750 micrograms kg-1 rapidly increased plasma TNF activity to a maximum at 60 min after initiation of LPS infusion. By 180 min, TNF activity returned to baseline. Cats produce TNF in response to LPS in a manner similar to other species. Measurement of TNF activity in plasma or in LPS-stimulated whole blood are methods to further characterize the inflammatory response in feline diseases.
暴露于脂多糖（LPS）的猫腹膜渗出细胞（PEC）的上清液产生的肿瘤坏死因子（TNF）活性显著高于（P<0.05）暴露于培养基的细胞的上清液。全血与10微克/毫升LPS孵育2小时后获得体外LPS反应。静脉注射LPS（750微克/千克）后，血浆TNF活性在LPS注射开始后60分钟迅速升至最高。到180分钟时，TNF活性恢复到基线水平。猫对LPS产生TNF的方式与其他物种相似。测量血浆或LPS刺激的全血中的TNF活性是进一步表征猫科疾病炎症反应的方法。
