Rzepecki R, Szopa J
Institute of Biochemistry, Wrocław University, Poland.
Acta Biochim Pol. 1995;42(2):177-82.
Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 micrograms IgG per 1 x 10(6) cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Preincubation of cell nuclei of White bush with IgG generates longer DNA fragments than in controls. Involvement of the 32 kDa endonuclease or an endonuclease-65 kDa protein complex from the nuclear matrix in replication or structural organisation of replication is considered.
抗32 kDa核酸内切酶免疫球蛋白可抑制DNA合成。我们观察到，低浓度的免疫球蛋白（每1×10⁶个细胞核约50微克免疫球蛋白）可暂时抑制DNA合成。这种抑制仅涉及与核基质结合的DNA（与分离的核基质相关）的合成。用免疫球蛋白对白布什细胞核进行预孵育会产生比对照更长的DNA片段。人们认为，来自核基质的32 kDa核酸内切酶或65 kDa核酸内切酶蛋白复合物参与了复制或复制的结构组织。
