Kozubek A
Institute of Biochemistry, University of Wrocław, Poland.
Acta Biochim Pol. 1995;42(2):247-51.
Polycratic reversed-phase high-performance liquid chromatography (RPHPLC) was used for estimation of the octanol/water partition coefficient for three highly hydrophobic long chain orcinol homologs. The homologs studied (C 15:0, C 17:0 and C 19:0) showed high preference for hydrophobic phase as evidenced by their high octanol/water partition coefficient (log Po/w) values of 7.02-7.74; 8.71-9.47 and 10.49-11.32 for the 95% prediction interval, respectively. Experimentally estimated values were compared with log P values calculated with the use of several fragmental systems. The experimental values of log Po/w are in best agreement to those calculated with the use of the Klopman system (Klopman, G., Namboodiri, K. & Schochet, M., 1985, J. Comput. Chem. 6, 28-38). The lack of appropriate standard compounds with known log Po/w in the range over 6 markedly affected the accuracy of experimental determinations.
采用多元反相高效液相色谱法（RPHPLC）测定了三种高度疏水的长链苔黑酚同系物的正辛醇/水分配系数。所研究的同系物（C 15:0、C 17:0和C 19:0）对疏水相表现出高度偏好，这从它们的高正辛醇/水分配系数（log Po/w）值可以看出，95%预测区间的log Po/w值分别为7.02 - 7.74、8.71 - 9.47和10.49 - 11.32。将实验估计值与使用几种碎片系统计算得到的log P值进行了比较。log Po/w的实验值与使用克洛普曼系统（Klopman, G., Namboodiri, K. & Schochet, M., 1985, J. Comput. Chem. 6, 28 - 38）计算得到的值最为吻合。在log Po/w超过6的范围内缺乏合适的已知标准化合物，这显著影响了实验测定的准确性。
