Sobczak K, Kozłowski P, Krzyzosiak W J
Laboratory of Cancer Genetics, Polish Academy of Sciences, Poznań, Poland.
Acta Biochim Pol. 1995;42(3):363-6.
The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be done even in a reaction volume as low as 1 microliter. The recommended volume, 2.5 microliters or 5 microliters, allows significant savings in the laboratory budget especially for laboratories which use PCR frequently and on a large scale.
聚合酶链反应（PCR）条件已得到优化，以使该过程更快且更经济。当要扩增短DNA片段时，变性、退火和延伸步骤的时间可短至各1秒，且PCR产物的产量仍然很高，足以用于多种类型的分析。PCR甚至可以在低至1微升的反应体积中进行。推荐的体积为2.5微升或5微升，这尤其能为经常大量使用PCR的实验室大幅节省实验室预算。
