Packter N M, Olukoshi E R
Department of Biochemistry and Molecular Biology, The University of Leeds, Leeds LS2 9JT, UK.
Arch Microbiol. 1995 Dec;164(6):420-7. doi: 10.1007/BF02529740.
Triacylglycerol is accumulated by Streptomyces spp. when grown in submerged culture. Ultrastructural studies using transmission electron microscopy (TEM), staining and freeze-fracture/freeze-etch procedures, and light microscopy confirmed the accumulation of neutral lipid by S. lividans and S. coelicolor during the stationary phase and its storage within membrane-bound globular structures within the cytoplasm. These structures were of various sizes and occupied up to approximately 80% of the total cell volume at that time. There was no evidence of such material within cells examined during the early exponential phase of growth. The globules visualised by TEM were electron-transparent since they comprised lipids containing saturated fatty acids that did not react with osmium tetroxide. The globules appeared to be bounded by a single membrane.
当链霉菌属在深层培养中生长时会积累三酰甘油。使用透射电子显微镜（TEM）、染色以及冷冻断裂/冷冻蚀刻程序和光学显微镜进行的超微结构研究证实，在稳定期，淡紫灰链霉菌和天蓝色链霉菌会积累中性脂质，并将其储存在细胞质中膜结合的球状结构内。这些结构大小各异，在那个时候占据了细胞总体积的约80%。在生长的指数早期阶段检查的细胞内没有这种物质的迹象。通过TEM观察到的小球是电子透明的，因为它们由含有不与四氧化锇反应的饱和脂肪酸的脂质组成。这些小球似乎由单层膜包围。
