Christiansen K, Carlsen J
Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark.
Cell Signal. 1995 Aug;7(6):583-9. doi: 10.1016/0898-6568(95)00032-k.
Isolated insulin receptors were inserted into hepatocytes by fusion with receptor-lysophospholipid micelles. The number of receptors was increased approximately two times as monitored by insulin binding experiments. Addition of exogenous receptors resulted in increased receptor autophosphorylation. The exogenous added receptors could be coupled to the intracellular effector system as shown by increased phosphorylation of the insulin receptor substrate 1, IRS-1, and by increased glycogen synthesis. The co-incorporated lysophospholipid was partly metabolized to phospholipid. This method for introducing insulin receptors into cells represents a novel system to study the pathway of insulin signalling and may provide information as to the role of variation of receptor number in insulin action in cellular metabolism of specific cells.
通过与受体 - 溶血磷脂微团融合，将分离出的胰岛素受体插入肝细胞中。通过胰岛素结合实验监测，受体数量增加了约两倍。添加外源性受体导致受体自身磷酸化增加。如胰岛素受体底物1（IRS - 1）磷酸化增加以及糖原合成增加所示，添加的外源性受体可与细胞内效应系统偶联。共掺入的溶血磷脂部分代谢为磷脂。这种将胰岛素受体引入细胞的方法代表了一种研究胰岛素信号传导途径的新系统，并且可能提供有关受体数量变化在特定细胞的细胞代谢中胰岛素作用的信息。
