Périchon R, Bourre J M
Laboratoire de Neurotoxiologie, INSERM U26, Hôpital Fernand Widal, Paris, France.
Biochimie. 1995;77(4):288-93. doi: 10.1016/0300-9084(96)88138-7.
Liver peroxisomal beta-oxidation activity on stearate, oleate, linoleate and alpha-linolenate was investigated as a function of age in two mouse strains. Each fatty acid showed a similar beta-oxidation activity pattern with age characterized by a rapid increase (200%) from day 2 to 20 followed by a dramatic weaning-related decrease (70%) from day 20 to 22. There was a new increase (260%) from day 22 to 75, then a plateau up to day 300 days, and finally an age-related decrease (70%) from day 300 to 540. Oleic, linoleic and alpha-linolenic acids were respectively 5-, 7.5- and 9-fold more degraded than stearic acid. Catalase specific activity showed the same age-related pattern as fatty acid beta-oxidation. Both mouse strains showed the age-related decreases. The longer-lived strain exhibited higher activity for both peroxisomal beta-oxidation and catalase and the rate of decrease of these two activities during aging was the same for both strains. The catalase/alpha-linolenic acid beta-oxidation ratio was constant during adulthood and aging. These results suggest that peroxisomal beta-oxidation and catalase activities are closely related throughout and implications for long-chain and very long-chain fatty acid metabolism, maintenance of membrane fatty acid composition and anti-oxidant status during aging are discussed.
在两个小鼠品系中，研究了肝脏过氧化物酶体对硬脂酸、油酸、亚油酸和α-亚麻酸的β-氧化活性随年龄的变化。每种脂肪酸的β-氧化活性模式随年龄变化相似，其特征为从第2天到第20天迅速增加（200%），随后在第20天到第22天因断奶而急剧下降（70%）。从第22天到第75天又有新的增加（260%），然后直到第300天保持平稳，最后从第300天到第540天随年龄下降（70%）。油酸、亚油酸和α-亚麻酸的降解速度分别比硬脂酸高5倍、7.5倍和9倍。过氧化氢酶比活性呈现出与脂肪酸β-氧化相同的年龄相关模式。两个小鼠品系均表现出与年龄相关的下降。寿命较长的品系在过氧化物酶体β-氧化和过氧化氢酶方面均表现出较高的活性，并且这两种活性在衰老过程中的下降速率在两个品系中相同。在成年期和衰老过程中，过氧化氢酶/α-亚麻酸β-氧化比值保持恒定。这些结果表明，过氧化物酶体β-氧化和过氧化氢酶活性在整个过程中密切相关，并讨论了其对长链和极长链脂肪酸代谢、衰老过程中膜脂肪酸组成的维持以及抗氧化状态的影响。
