Gonda T, Akiyoshi H, Ichihara K
Institute of Experimental Animals, Shimane Medical University, Izumo, Japan.
J Smooth Muscle Res. 1995 Aug;31(4):153-62. doi: 10.1540/jsmr.31.153.
Three-dimensional structures of the nerves of the guinea-pig gallbladder, after histochemical demonstration of the acetylcholinesterase activity and HCl hydrolysis-collagenase digestion, were examined by scanning electron microscope. HCl-collagenase digestion facilitated easy identification of silver- and gold-intensified acetylcholinesterase-positive nerve fibers at a high accelerating voltage (25 kV), due to their strong reflection image. Ganglia were either triangular or ovoidal in shape. Dense para- and peri-vascular nerve fibers occurred around the cystic artery. There were a few intramuscular nerve fibers with varicosity-like structures among smooth muscle bundles. Dense branched and tapering nerve fibers with varicosities in the lamina propria mucosae were closely attached to epithelial cells. The acetylcholinesterase-positive fibers in the lamina propria and peri- and para-vascular nerves, and fewer positive fibers in the smooth muscle layer probably represent cholinergic nerves involved in the concentration of biliary compounds and lesser in the motor function of the smooth muscle.
在对豚鼠胆囊神经的乙酰胆碱酯酶活性进行组织化学显示并经盐酸水解 - 胶原酶消化后，利用扫描电子显微镜检查其三维结构。由于盐酸 - 胶原酶消化后的神经纤维具有强烈的反射图像，在高加速电压（25 kV）下便于识别银和金增强的乙酰胆碱酯酶阳性神经纤维。神经节呈三角形或椭圆形。胆囊动脉周围出现密集的血管旁和血管周围神经纤维。在平滑肌束之间有一些具有曲张样结构的肌内神经纤维。固有层中密集的分支且逐渐变细的有曲张的神经纤维紧密附着于上皮细胞。固有层、血管周围和血管旁神经中的乙酰胆碱酯酶阳性纤维，以及平滑肌层中较少的阳性纤维可能代表参与胆汁化合物浓缩的胆碱能神经，而在平滑肌运动功能中作用较小。
