Ruben L N, Gwinnell E M
J Exp Zool. 1977 Apr;200(1):137-42. doi: 10.1002/jez.1402000116.
The effects of hydrocortisone (HC) and bacterial lipopolysaccharide (LPS) have been tested separately and in combination, on the antigen-binding response of splenocytes of the newt. Triturus viridescens immunized with horse erythrocytes (HRBC). The timing of the presentation of each was made relative to when challenge with either low (0.0025%) or high (25%) doses of the immunogen (HRBC) was effected. Our results showed inhibition of both low and high dose responses by HC and additionally suggested that the helper-cell population of the newt is HC-sensitive. LPS given four days prior to antigen challenge initiated the generation of antigen-binding cells which enhanced the low, but not the high dose response. The use of LPS in conjunction with HC provided suggestive evidence that those LPS stimulated cells which had enhanced the low immunogen dose antigen-binding cell response were HC-resistant. Thus LPS may stimulate a non-helper cell population in the newt which is HC-resistant. The use of these two immunologic probes has provided supportive evidence on the heterogeneity of the lymphoid population of the newt, Triturus viridescens.
已经分别测试了氢化可的松（HC）和细菌脂多糖（LPS）以及二者联合使用对用马红细胞（HRBC）免疫的绿螈脾脏细胞抗原结合反应的影响。每次给予药物的时间相对于用低剂量（0.0025%）或高剂量（25%）免疫原（HRBC）进行攻击的时间。我们的结果显示，HC对低剂量和高剂量反应均有抑制作用，此外还表明绿螈的辅助性细胞群体对HC敏感。在抗原攻击前四天给予LPS可启动抗原结合细胞的产生，从而增强低剂量而非高剂量反应。将LPS与HC联合使用提供了提示性证据，表明那些增强低剂量免疫原抗原结合细胞反应的LPS刺激细胞对HC具有抗性。因此，LPS可能刺激绿螈体内对HC具有抗性的非辅助性细胞群体。使用这两种免疫探针为绿螈（Triturus viridescens）淋巴样群体的异质性提供了支持性证据。
