Liere K, Kestermann M, Müller U, Link G
University of Bochum, Germany.
Curr Genet. 1995 Jul;28(2):128-30. doi: 10.1007/BF00315778.
A 1887-nucleotide chloroplast-DNA region from Arabidopsis thaliana was analyzed. It contains the conserved genes psbA for the precursor of the D1 reaction-centre protein of photosystem II, trnH for tRNAHis, and rps19' for the 6.8-kDa protein of the small ribosomal subunit. Northern hybridization and RNase protection experiments suggest co-transcription of a minor RNA fraction over the full lengths of psbA and the preceding trnK-UUU gene, but not including downstream trnH sequences. In front of the mapped 5' end of the major 1.2-kb psbA transcript is a DNA region that shows the typical architecture of a psbA promoter, consisting of the prokaryotic-type '-35' and '-10' elements as well as the eukaryotic-type 'TATA' motif. The common 3' end of psbA transcripts seems to be located immediately after a stem-loop structure downstream from the coding region.
对拟南芥中一段1887个核苷酸的叶绿体DNA区域进行了分析。它包含保守基因，即光系统II的D1反应中心蛋白前体的psbA、tRNAHis的trnH以及小核糖体亚基6.8 kDa蛋白的rps19'。Northern杂交和RNase保护实验表明，在psbA全长以及前面的trnK-UUU基因上，有一小部分RNA共同转录，但不包括下游的trnH序列。在主要的1.2 kb psbA转录本定位的5'端之前，有一个DNA区域，它显示出psbA启动子的典型结构，由原核类型的“-35”和“-10”元件以及真核类型的“TATA”基序组成。psbA转录本的共同3'端似乎位于编码区下游的一个茎环结构之后。
