Zurawski G, Bottomley W, Whitfeld P R
Nucleic Acids Res. 1984 Aug 24;12(16):6547-58. doi: 10.1093/nar/12.16.6547.
This work describes the organization, at the nucleotide sequence level, of genes flanking the junctions of the large single copy regions and the inverted repeats of Spinacia oleracea (spinach) and Nicotiana debneyi chloroplast DNAs. In both genomes, trnH1, the gene for tRNA-His(GUG) is located at the extremity of the large single copy region 3' to psbA, the gene for the 35 kd Photosystem 2 protein. Both psbA and trnH1 are transcribed towards the inverted repeat. In spinach, the first 48 codons of rps19, the gene for the chloroplast ribosomal protein S19, lie in the inverted repeat and the last 44 codons lie in the large single copy region at the end opposite to that carrying trnH1. The gene for a protein homologous to the E. coli ribosomal protein L2, rp12, is in the inverted repeat immediately 5' to rps19 and, like rps19, is transcribed towards the large single copy region. In N. debneyi, but not in spinach, rp12 is interrupted by a 666 bp insertion. The gene for tRNA-lle(CAT), trnl1, is located in the inverted repeats of spinach and N. debneyi, 5' to rp12 and is transcribed in the same direction as rp12.
这项工作描述了菠菜（Spinacia oleracea）和德氏烟草（Nicotiana debneyi）叶绿体DNA中，大单拷贝区域与反向重复序列交界处两侧基因在核苷酸序列水平上的组织情况。在这两个基因组中，tRNA-His(GUG)的基因trnH1位于大单拷贝区域的末端，在编码35kd光系统2蛋白的基因psbA的3'端。psbA和trnH1均朝着反向重复序列转录。在菠菜中，叶绿体核糖体蛋白S19的基因rps19的前48个密码子位于反向重复序列中，最后44个密码子位于大单拷贝区域中与携带trnH1的一端相对的另一端。与大肠杆菌核糖体蛋白L2同源的一种蛋白质的基因rp12，位于rps19紧挨着的5'端的反向重复序列中，并且与rps19一样，朝着大单拷贝区域转录。在德氏烟草中，但不在菠菜中，rp12被一个666bp的插入序列打断。tRNA-Ile(CAT)的基因trnI1位于菠菜和德氏烟草的反向重复序列中，在rp12的5'端，并且与rp12转录方向相同。
