Faulds C B, Williamson G
Department of Food Molecular Biochemistry, Institute of Food Research, UK.
Appl Microbiol Biotechnol. 1995 Nov;43(6):1082-7. doi: 10.1007/BF00166929.
Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of ferulic acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of ferulic acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of ferulic acid.
当与绿色木霉木聚糖酶一起孵育时，黑曲霉的阿魏酸酯酶（FAE-III）能有效地从小麦麸皮制剂中释放出阿魏酸（孵育5小时后，阿魏酸的总释放量最高可达95%）。FAE-III自身也能释放阿魏酸，但释放量比在木聚糖酶（2单位）存在时低近24倍。在0.1单位至1.3单位之间，阿魏酸的释放量与FAE-III的浓度成正比，但低水平木聚糖酶（0.1单位）的存在使阿魏酸的释放量增加了6倍。FAE-III对小麦麸皮的作用不影响总糖的释放，但酯酶的存在提高了木聚糖酶作用的表观终产物（木糖和木二糖）的释放速率。结果表明，FAE-III和木聚糖酶共同作用，分解阿魏酰化的植物细胞壁多糖，从而获得高产率的阿魏酸。
