Zhang S, Holmes T C, DiPersio C M, Hynes R O, Su X, Rich A
Department of Biology 68-233, Massachusetts Institute of Technology, Cambridge 02139-4307, USA.
Biomaterials. 1995 Dec;16(18):1385-93. doi: 10.1016/0142-9612(95)96874-y.
A new class of ionic self-complementary oligopeptides is described, two members of which have been designated RAD16 and EAK16. These oligopeptides consist of regular repeats of alternating ionic hydrophilic and hydrophobic amino acids and associate to form stable beta-sheet structures in water. The addition of buffers containing millimolar amounts of monovalent salts or the transfer of a peptide solution into physiological solutions results in the spontaneous assembly of the oligopeptides into a stable, macroscopic membranous matrix. The matrix is composed of ordered filaments which form porous enclosures. A variety of mammalian cell types are able to attach to both RAD16 and EAK16 membranous matrices. These matrices provide a novel experimental system for analysing mechanisms of in vitro cell attachment and may have applications in in vivo studies of tissue regeneration, tissue transplantation and would healing.
本文描述了一类新型的离子自互补寡肽，其中两个成员被命名为RAD16和EAK16。这些寡肽由离子亲水性和疏水性氨基酸交替的规则重复序列组成，在水中缔合形成稳定的β-折叠结构。添加含有毫摩尔量单价盐的缓冲液或将肽溶液转移到生理溶液中会导致寡肽自发组装成稳定的宏观膜状基质。该基质由形成多孔包封的有序细丝组成。多种哺乳动物细胞类型能够附着于RAD16和EAK16膜状基质。这些基质为分析体外细胞附着机制提供了一个新的实验系统，并且可能在组织再生、组织移植和伤口愈合的体内研究中有应用。
