Marjan Tuba, Lafuente-Gómez Nuria, Rampal Akaansha, Mooney David J, Peyton Shelly R, Qazi Taimoor H
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA; email:
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA; email:
Annu Rev Biomed Eng. 2025 May;27(1):185-209. doi: 10.1146/annurev-bioeng-120823-020209. Epub 2025 Jan 28.
Biochemical signals in native tissue microenvironments instruct cell behavior during many biological processes ranging from developmental morphogenesis and tissue regeneration to tumor metastasis and disease progression. The detection and characterization of these signals using spatial and highly resolved quantitative methods have revealed their existence as matricellular proteins in the matrisome, some of which are bound to the extracellular matrix while others are freely diffusing. Including these biochemical signals in engineered biomaterials can impart enhanced functionality and native-like complexity, ultimately benefiting efforts to understand, model, and treat various diseases. In this review, we discuss advances in characterizing, mimicking, and harnessing biochemical signals in developing advanced engineered biomaterials. An overview of the diverse forms in which these biochemical signals exist and their effects on intracellular signal transduction is also provided. Finally, we highlight the application of biochemically complex biomaterials in the three broadly defined areas of tissue regeneration, immunoengineering, and organoid morphogenesis.
在从发育形态发生、组织再生到肿瘤转移和疾病进展等许多生物过程中，天然组织微环境中的生化信号指导细胞行为。使用空间和高分辨率定量方法对这些信号进行检测和表征，揭示了它们作为基质组中基质细胞蛋白的存在，其中一些与细胞外基质结合，而另一些则自由扩散。将这些生化信号纳入工程生物材料中可以赋予其增强的功能和类似天然的复杂性，最终有利于理解、模拟和治疗各种疾病的研究工作。在这篇综述中，我们讨论了在开发先进工程生物材料过程中表征、模拟和利用生化信号方面的进展。还概述了这些生化信号存在的多种形式及其对细胞内信号转导的影响。最后，我们重点介绍了生物化学复杂生物材料在组织再生、免疫工程和类器官形态发生这三个广义定义领域中的应用。
