Tassanakajon A, Pongsomboon S, Rimphanitchayakit V, Jarayabhand P, Boonsaeng V
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok Thailand.
Mol Mar Biol Biotechnol. 1997 Jun;6(2):110-5.
Random amplified polymorphic DNA (RAPD) analysis was used to amplify the genome of black tiger prawns (Penaeus monodon) to detect DNA markers and assess the utility of the RAPD method for investigating genetic variation in wild P. monodon in Thailand. A total of 200 ten-base primers were screened, and 84 primers yielded amplification products. Six positive primers that gave highly reproducible RAPD patterns were selected for the analysis of three geographically different samples of Thai P. monodon. A total of 70 reproducible RAPD fragments ranging in size from 200 to 2000 bp were scored, and 40 fragments (57%) were polymorphic. The RAPD analysis of broodstocks from three different locales, Satun-Trang, Trat, and Angsila, revealed different levels of genetic variability among the samples. The percentages of polymorphic bands were 48% and 45% in Satun-Trang and Trat, respectively, suggesting a high genetic variability of the two samples to be used in selective breeding programs. Only 25% polymorphic bands were found in the Angsila sample, indicating the lowest polymorphic level among the three samples examined. Primer 428 detected a RAPD marker that was found only in P. monodon originating from Satun-Trang, suggesting the potential use of this marker as a population-specific marker in this species.
随机扩增多态性DNA（RAPD）分析被用于扩增黑虎虾（斑节对虾）的基因组，以检测DNA标记，并评估RAPD方法在研究泰国野生斑节对虾遗传变异方面的实用性。总共筛选了200条十碱基引物，其中84条引物产生了扩增产物。选择了6条能产生高度可重复RAPD图谱的阳性引物，用于分析来自泰国三个地理区域的斑节对虾样本。总共记录了70条大小在200至2000 bp之间的可重复RAPD片段，其中40条片段（57%）具有多态性。对来自三个不同地点（沙敦-董里、达叻和安帕瓦）的亲虾进行的RAPD分析显示，样本之间的遗传变异水平不同。沙敦-董里和达叻样本中的多态性条带百分比分别为48%和45%，这表明这两个样本在选择性育种计划中有很高的遗传变异性。在安帕瓦样本中仅发现25% 的多态性条带，表明在所检测的三个样本中其多态性水平最低。引物428检测到一个RAPD标记，该标记仅在来自沙敦-董里的斑节对虾中发现，这表明该标记有可能作为该物种的种群特异性标记。
