Yoshida K, Sumi S, Honda M, Hosoya Y, Yano M, Arai K, Ueda Y
Department of Urology, Dokkyo University School of Medicine, Tochigi, Japan.
J Chromatogr B Biomed Appl. 1995 Oct 6;672(1):45-51. doi: 10.1016/0378-4347(95)00199-s.
Differences between human renal cortex and human renal cell carcinoma (RCC) in asparagine (Asn)-linked sugar chain structures of gamma-glutamyltransferase (GGT) were investigated by using a serial lectin affinity chromatographic technique. The relative amounts of GGT which passed through the concanavalin A (Con A) column but bound to the phytohaemagglutinin E column, were significantly decreased in RCC, but there were significant increases in the relative amounts of GGT which bound weakly to the Con A column and passed through the pea lectin (PSA) column, and bound strongly to the Con A column and bound to the wheat germ agglutinin column in RCC compared with those of the normal renal cortex. A significant correlation was observed in RCC between nuclear grade and relative amount of GGT which bound weakly to the Con A column and passed through the PSA column. The findings indicate that Asn-linked sugar chain structures are altered in RCC and suggest that studies of qualitative differences of sugar chain structures of GGT might lead to a useful diagnostic tool for human RCC.
采用一系列凝集素亲和色谱技术，研究了人肾皮质与人肾细胞癌（RCC）中γ-谷氨酰转移酶（GGT）天冬酰胺（Asn）连接糖链结构的差异。在RCC中，通过伴刀豆球蛋白A（Con A）柱但与植物血凝素E柱结合的GGT相对量显著降低，但与正常肾皮质相比，RCC中与Con A柱弱结合并通过豌豆凝集素（PSA）柱的GGT相对量以及与Con A柱强结合并与麦胚凝集素柱结合的GGT相对量均显著增加。在RCC中，核分级与与Con A柱弱结合并通过PSA柱的GGT相对量之间存在显著相关性。这些发现表明RCC中Asn连接糖链结构发生了改变，并提示对GGT糖链结构定性差异的研究可能会产生一种用于人类RCC的有用诊断工具。
