Sumi S, Arai K, Kitahara S, Yoshida K
Department of Urology, Dokkyo University School of Medicine, Tochigi, Japan.
J Chromatogr B Biomed Sci Appl. 1999 Apr 30;727(1-2):9-14. doi: 10.1016/s0378-4347(99)00069-9.
Differences between prostate carcinoma (PCA) and benign prostatic hyperplasia (BPH) in asparagine (N)-linked sugar-chain structures of prostate-specific antigen (PSA) were investigated using serial lectin affinity chromatography. The amounts of PSA passing through columns of concanavalin A (Con A), phytohaemagglutinin E4 (PHA-E4) and PHA-L4 were significantly greater for PCA-derived PSA than BPH. We propose that the sugar moiety structure of PSA which is increased in PCA is a multiantennary complex type with branched N-acetylglucosamine beta(1->4) mannose. We suggest that N-linked sugar chains in PSA are altered during oncogenesis in the human prostate and may serve as diagnostic tools for PCA.
使用连续凝集素亲和色谱法研究了前列腺癌（PCA）和良性前列腺增生（BPH）中前列腺特异性抗原（PSA）的天冬酰胺（N）-连接糖链结构的差异。PCA来源的PSA通过伴刀豆球蛋白A（Con A）、植物血凝素E4（PHA-E4）和PHA-L4柱的PSA量明显高于BPH。我们提出，PCA中增加的PSA糖部分结构是一种具有分支N-乙酰葡糖胺β(1->4)甘露糖的多天线复合型。我们认为，PSA中的N-连接糖链在人类前列腺肿瘤发生过程中发生改变，可能作为PCA的诊断工具。
