Yamaguchi M, Miyake A
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Eur J Endocrinol. 1996 Jan;134(1):123-7. doi: 10.1530/eje.0.1340123.
The effect of factors secreted from the pituitary on mouse placental lactogen I (mPL-I) and mPL-II secretion in vitro was examined. Co-culture of mouse placental cells from day 7 of pregnancy with the pituitary cells of the mother significantly stimulated mPL-I secretion but did not regulate mPL-II secretion. The effect on mPL-I secretion was dependent on the number of pituitary cells. The conditioned medium of pituitary cells also significantly stimulated mPL-I secretion but did not regulate mPL-II secretion. The stimulatory effect of mPL-I secretion was dependent on the volume of the conditioned medium. The number of cells containing mPL-I assessed by immunocytochemistry was increased by the co-culture in a cell number-dependent manner. Northern blot analysis for mPL-I indicated that treatment of placental cells with the pituitary-conditioned medium results in an increase of mPL-I gene expression. These findings suggest that factors secreted from the pituitary directly regulate mPL-I secretion, but not mPL-II secretion, before midpregnancy in vivo.
研究了垂体分泌的因子对体外培养的小鼠胎盘催乳素I（mPL-I）和mPL-II分泌的影响。将妊娠第7天的小鼠胎盘细胞与母体垂体细胞共培养，显著刺激了mPL-I的分泌，但对mPL-II的分泌没有调节作用。对mPL-I分泌的影响取决于垂体细胞的数量。垂体细胞的条件培养基也显著刺激了mPL-I的分泌，但对mPL-II的分泌没有调节作用。mPL-I分泌的刺激作用取决于条件培养基的体积。通过免疫细胞化学评估，含有mPL-I的细胞数量因共培养而以细胞数量依赖的方式增加。mPL-I的Northern印迹分析表明，用垂体条件培养基处理胎盘细胞会导致mPL-I基因表达增加。这些发现表明，在体内妊娠中期之前，垂体分泌的因子直接调节mPL-I的分泌，而不调节mPL-II的分泌。
