Yamaguchi M, Sawada K, Miyake A
Osaka University Medical School, Japan.
J Endocrinol Invest. 1996 Jul-Aug;19(7):415-21. doi: 10.1007/BF03349885.
To determine whether lipopolysaccharides (LPS) regulate mouse placental lactogen-I (mPL-I), mPL-II, and mouse GHRF (mGHRF) secretion, mouse placental tissue from days 7, 9, and 12 of pregnancy was dispersed with collagenase and the purified trophoblast cells were cultured in a serum-free medium with or without LPS for 5 days. LPS significantly inhibited mPL-II secretion by cells from days 9 and 12 of pregnancy. However, LPS did not affect mPL-II secretion by cells from day 7 of pregnancy, mPL-I secretion by cells from days 7 and 9 of pregnancy, or mGHRF secretion by cells from day 12 of pregnancy. The inhibitory effect of LPS on mPL-II secretion by cells from day 12 of pregnancy was dose-dependent. Steady-state levels of mPL-II mRNA were significantly reduced by incubation of placental cells from day 12 of pregnancy with LPS. The inhibitory effect of LPS on mPL-II secretion was abolished by the addition of antibodies to IL-1 alpha and IL-6. These findings suggest that LPS selectively inhibit mPL-II secretion, at least partly through increases in IL-1 and IL-6 production, after midpregnancy.
为了确定脂多糖（LPS）是否调节小鼠胎盘催乳素-I（mPL-I）、mPL-II和小鼠生长激素释放因子（mGHRF）的分泌，将妊娠第7、9和12天的小鼠胎盘组织用胶原酶分散，纯化的滋养层细胞在含或不含LPS的无血清培养基中培养5天。LPS显著抑制妊娠第9天和12天细胞的mPL-II分泌。然而，LPS不影响妊娠第7天细胞的mPL-II分泌、妊娠第7天和9天细胞的mPL-I分泌或妊娠第12天细胞的mGHRF分泌。LPS对妊娠第12天细胞mPL-II分泌的抑制作用呈剂量依赖性。用LPS孵育妊娠第12天的胎盘细胞可显著降低mPL-II mRNA的稳态水平。添加抗IL-1α和IL-6抗体可消除LPS对mPL-II分泌的抑制作用。这些发现表明，妊娠中期后，LPS至少部分通过增加IL-1和IL-6的产生来选择性抑制mPL-II分泌。
