Kobe A, von Ahlen M, Fries R
Institut für Anatomie, Physiologie und Hygiene, Haustiere der Universität Bonn.
Berl Munch Tierarztl Wochenschr. 1996 Jan;109(1):14-7.
This study describes the influence of bioptivet GB on minimum inhibitory concentrations (MIC) for furazolidone of the intestinal E. coli flora of young broiler chickens after prophylactic treatment. From day 6 until day 15 one group of 50 birds received a diet containing 326 ppm furazolidone, another group of 75 birds served as non medicated control. Investigated E. coli had been isolated from cloacal swabs and from caecal contents. MIC of 1581 E. coli strains were determined by agar dilution test. MIC of furazolidone for the investigated strains ranged from 2 micrograms/ml to 64 micrograms/ml. For classification as "resistant" or "susceptible" limits of 16 micrograms/ml and 8 micrograms/ml respectively were used. Strains obtained from undosed birds mainly had MIC values of 4 micrograms/ml or 8 micrograms/ml, i.e. two or three times higher than MIC of E. coli ATCC 25 922, MIC values of 16 micrograms/ml or more were recorded only among isolates obtained from chickens which had received the drug. Administration of bioptivet GB resulted in a statistically significant increase in the average MIC. Statistically higher average MIC were recorded among isolates from cloacal swabs only during application of the drug. For strains from caecal contents, the effect became obvious only at the end of the experiment.
本研究描述了预防性治疗后，生物益生素GB对幼龄肉鸡肠道大肠杆菌菌群对呋喃唑酮的最低抑菌浓度（MIC）的影响。从第6天到第15天，一组50只鸡饲喂含326 ppm呋喃唑酮的日粮，另一组75只鸡作为未用药对照组。所研究的大肠杆菌是从泄殖腔拭子和盲肠内容物中分离得到的。采用琼脂稀释法测定了1581株大肠杆菌的MIC。所研究菌株对呋喃唑酮的MIC范围为2微克/毫升至64微克/毫升。分别以16微克/毫升和8微克/毫升作为“耐药”或“敏感”的分类界限。从未给药鸡中分离得到的菌株主要MIC值为4微克/毫升或8微克/毫升，即比大肠杆菌ATCC 25922的MIC高两到三倍，仅在从给药鸡中分离得到的菌株中记录到16微克/毫升或更高的MIC值。施用生物益生素GB导致平均MIC有统计学意义的升高。仅在用药期间，从泄殖腔拭子分离得到的菌株中记录到统计学上更高的平均MIC。对于来自盲肠内容物的菌株，这种影响仅在实验结束时才变得明显。
