Lee K O, Lee K P
Department of Molecular Genetics and Biochemistry Seoul Medical Science Institute, Seoul Clinical Laboratories, Korea.
J Korean Med Sci. 1995 Aug;10(4):243-9. doi: 10.3346/jkms.1995.10.4.243.
Hypervariable tandem repetitive regions in human DNA are proving to be increasingly useful for genetic analysis in humans. We chose four single locus probes (SLP; MS1, MS43, MS8 and g3) for a validation test among Koreans. The specimens were from 216 unrelated individuals and 33 paternity inclusion families. Extracted DNA from EDTA blood was restricted by Hinfl and electrophoresed in 0.7% agarose gel, transferred and hybridized with chemiluminescent probes. Heterozygosity was over 90% by all of the probes. Total numbers of unassignable mutant bands from 33 paternity inclusion cases were 5, and the highest mutation rate was determined in probe MS1(0.045). The probability of having the same DNA band between two unrelated individuals was 5.7 x 10(-10) when four SLPs were used at the same time. The data presented here on allele frequencies and mutation rates provide preliminary data supporting the validity of these probes in paternity analysis and forensic investigators in the Korean population.
人类DNA中的高变串联重复区域已被证明在人类基因分析中越来越有用。我们选择了四个单基因座探针（SLP；MS1、MS43、MS8和g3）在韩国人群中进行验证测试。样本来自216名无关个体和33个父权肯定家庭。从EDTA抗凝血中提取的DNA用Hinfl酶切，在0.7%的琼脂糖凝胶中电泳，转移后与化学发光探针杂交。所有探针的杂合度均超过90%。33个父权肯定案例中无法确定来源的突变带总数为5条，探针MS1的突变率最高（0.045）。同时使用四个SLP时，两个无关个体之间出现相同DNA条带的概率为5.7×10^(-10)。这里给出的等位基因频率和突变率数据为这些探针在韩国人群的父权分析和法医调查中的有效性提供了初步数据支持。
