Vokuev Mikhail, Melekhin Artem, Frolova Anastasia, Bairov Anton, Rodin Igor, Tishchenko Victor
Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
Federal Centre for Animal Health, 600901 Vladimir, Russia.
Food Chem X. 2024 Dec 10;25:102087. doi: 10.1016/j.fochx.2024.102087. eCollection 2025 Jan.
Metronidazole (MNZ) is one of the most commonly used antibiotics in the food industry. High levels in food can lead to the development of antimicrobial resistance in humans, so it is important to monitor its levels in food. In the context of legal proceedings, it is frequently necessary to re-examine samples after an extended period of time. It is therefore crucial to ensure that the analytes in question do not degrade during the storage period. In this study, HPLC-MS/MS approach was validated and used to analyze levels of MNZ and its hydroxy metabolite in chicken eggs during storage in the refrigerator (+4 °C) and in the freezer (-20 °C) for 3 months. An analyte solution was administered to hens to obtain eggs containing MNZ and hydroxymetronidazole (MNZ-OH). The dependence of the analyte content in eggs as a function of time after sampling was also investigated.
甲硝唑（MNZ）是食品工业中最常用的抗生素之一。食品中甲硝唑含量过高会导致人体产生抗菌耐药性，因此监测其在食品中的含量非常重要。在法律诉讼过程中，通常需要在较长时间后重新检测样品。因此，确保所讨论的分析物在储存期间不会降解至关重要。在本研究中，高效液相色谱-串联质谱法（HPLC-MS/MS）得到验证，并用于分析鸡蛋在冰箱（+4°C）和冷冻室（-20°C）中储存3个月期间甲硝唑及其羟基代谢物的含量。将分析物溶液注射到母鸡体内，以获得含有甲硝唑和羟基甲硝唑（MNZ-OH）的鸡蛋。还研究了采样后鸡蛋中分析物含量随时间的变化情况。
