Kohama Y, Tone H, Moriyama K, Arai S, Takae S, Tsujikawa K, Mimura T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Biol Pharm Bull. 1995 Oct;18(10):1315-9. doi: 10.1248/bpb.18.1315.
T lymphocyte unresponsiveness, induced in mice by a single gastric intubation of 0.2 ml cedar pollen extract (CPE, containing 4 micrograms protein/ml)/mouse daily for 3 to 28 consecutive days, was evaluated by the absence of a proliferative response of popliteal lymph node (PLN) T lymphocytes to CPE in vitro. T lymphocyte unresponsiveness increased with the period of gastric intubation of CPE and reached more than 80% of the control on day 28. The unresponsiveness to CPE was antigen-specific and T lymphocyte-mediated. In vitro CPE-specific T lymphocyte proliferation was significantly suppressed by intestinal intraepithelial lymphocytes (IELs) and hepatic mononuclear cells (MNCs), but not by spleen cells or PLN T lymphocytes from mice fed CPE for 28 days. The effector activity of IELs and MNCs was obviously antigen-specific. These results suggest that lymphocytes in the intestine and liver of mice fed CPE would be involved in the induction and maintenance of CPE-specific T lymphocyte unresponsiveness.
通过每天给小鼠单次胃内插管0.2 ml雪松花粉提取物（CPE，含4微克蛋白质/毫升），连续3至28天，诱导小鼠T淋巴细胞无反应性，并通过腘窝淋巴结（PLN）T淋巴细胞在体外对CPE无增殖反应来评估。T淋巴细胞无反应性随CPE胃内插管时间的延长而增加，在第28天达到对照的80%以上。对CPE的无反应性具有抗原特异性且由T淋巴细胞介导。肠道上皮内淋巴细胞（IELs）和肝单核细胞（MNCs）可显著抑制体外CPE特异性T淋巴细胞增殖，但喂食CPE 28天的小鼠的脾细胞或PLN T淋巴细胞则无此作用。IELs和MNCs的效应活性明显具有抗原特异性。这些结果表明，喂食CPE的小鼠肠道和肝脏中的淋巴细胞可能参与了CPE特异性T淋巴细胞无反应性的诱导和维持。
