Richman L K, Chiller J M, Brown W R, Hanson D G, Vaz N M
J Immunol. 1978 Dec;121(6):2429-34.
Specific immune unresponsiveness was induced in inbred mice (BDF1) by the administration of soluble ovalbumin (OVA) by gastric intubation. Anti-hapten (DNP) responses likewise were specifically diminished when animals were fed autologous carrier (OVA or keyhole limpet hemocyanin). Adoptive transfer of spleen cells demonstrated that the tolerant state could be maintained in irradiated recipient mice, and specific anergy could be transferred to normal recipient animals. Adoptive suppression was mediated by T lymphocytes, as demonstrated by nylon wool fractionation and susceptibility of the cells to anti-Thy 1.2 and complement. Transferred B cells had neither suppressive nor augmentative effects. Enteric administration of OVA also specifically diminished antigen-induced DNA synthesis of primed lymph node T cells, although suppressor cells were not identified in the lymph nodes per se.
通过胃插管给予可溶性卵清蛋白（OVA），在近交系小鼠（BDF1）中诱导了特异性免疫无反应性。当给动物喂食自体载体（OVA或钥孔戚血蓝蛋白）时，抗半抗原（DNP）反应同样会特异性减弱。脾细胞的过继转移表明，耐受状态可在经辐射的受体小鼠中维持，且特异性无反应性可转移至正常受体动物。如尼龙毛分级分离以及细胞对抗Thy 1.2和补体的敏感性所证明，过继抑制由T淋巴细胞介导。转移的B细胞既无抑制作用也无增强作用。经肠道给予OVA也特异性减弱了致敏淋巴结T细胞的抗原诱导的DNA合成，尽管在淋巴结本身未鉴定出抑制细胞。
