Fujita M, Hong K, Ito Y, Fujii R, Kariya K, Nishimuro S
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd., Kobe, Japan.
Biol Pharm Bull. 1995 Oct;18(10):1387-91. doi: 10.1248/bpb.18.1387.
Nattokinase is a new fibrinolytic enzyme which cleaves directly cross-linked fibrin in vitro. In this study, we investigated the thrombolytic effect of nattokinase on a thrombus in the common carotid artery of rat in which the endothelial cells of the vessel wall were injured by acetic acid. When a section of occluded vessel was stained for CD61 antigen by immunofluorescence utilizing a monoclonal antibody, the antigen was localized around the surface of the occluded blood vessels. This result suggests that the occlusive thrombosis was caused by platelet aggregation. In addition, thrombolysis with urokinase (UK; 50000 IU/kg, i.v.) or tissue plasminogen activator (tPA; 13300 IU/kg, i.v.) in our model was observed to restore the blood flow over a 60 min monitoring period. The results indicate that our chemically induced model is useful for screening and evaluating a thrombolytic agent. We evaluated the thrombolytic activity of nattokinase using this model and compared it with fibrino(geno)lytic enzyme, plasmin or elastase. On a molar basis, the recovery of the arterial blood flow with nattokinase, plasmin and elastase were 62.0 +/- 5.3%, 15.8 +/- 0.7% and 0%, respectively. The results indicate that the thrombolytic activity of nattokinase is stronger than that of plasmin or elastase in vivo.
纳豆激酶是一种新型纤溶酶，可在体外直接裂解交联纤维蛋白。在本研究中，我们研究了纳豆激酶对大鼠颈总动脉血栓的溶栓作用，该动脉血管壁内皮细胞已被乙酸损伤。当使用单克隆抗体通过免疫荧光对一段闭塞血管进行CD61抗原染色时，抗原定位于闭塞血管表面周围。这一结果表明闭塞性血栓形成是由血小板聚集引起的。此外，在我们的模型中，观察到用尿激酶（UK；50000 IU/kg，静脉注射）或组织纤溶酶原激活剂（tPA；13300 IU/kg，静脉注射）进行溶栓可在60分钟的监测期内恢复血流。结果表明，我们的化学诱导模型可用于筛选和评估溶栓剂。我们使用该模型评估了纳豆激酶的溶栓活性，并将其与纤维蛋白（原）溶解酶、纤溶酶或弹性蛋白酶进行了比较。以摩尔为基础，纳豆激酶、纤溶酶和弹性蛋白酶使动脉血流恢复的比例分别为62.0±5.3%、15.8±0.7%和0%。结果表明，纳豆激酶在体内的溶栓活性强于纤溶酶或弹性蛋白酶。
