Kaji T, Yamamoto C, Miyajima S, Suzuki M, Fujiwara Y, Sakamoto M, Koizumi F
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Biol Pharm Bull. 1995 Oct;18(10):1392-5. doi: 10.1248/bpb.18.1392.
Human, rabbit and rat aortic smooth muscle cells (hSMC, rSMC and A10 cells, respectively) were cultured with cadmium chloride and compared with human Chang liver cells to characterize the response of vascular smooth muscle cells to the metal. It was revealed that all tested vascular smooth muscle cells were markedly more sensitive to cadmium cytotoxicity than Chang liver cells. Cadmium accumulated more markedly in vascular smooth muscle cells than in Chang liver cells. After exposure to cadmium, metallothionein was induced in a concentration-dependent manner in Chang liver cells, it was constitutively high in hSMC, sensitively induced in rSMC and was constitutively low and induced within narrow limits in A10 cells. The intracellular content of reduced glutathione was greater and significantly enhanced by cadmium only in A10 cells. The present data suggest that vascular smooth muscle cells are, in general, sensitive to cadmium cytotoxicity without any species-related differences, mainly due to a higher accumulation of the metal within cells.
分别用人主动脉平滑肌细胞（hSMC）、兔主动脉平滑肌细胞（rSMC）和大鼠主动脉平滑肌细胞（A10细胞）与氯化镉共同培养，并与人类Chang肝细胞进行比较，以表征血管平滑肌细胞对该金属的反应。结果显示，所有受试的血管平滑肌细胞对镉细胞毒性的敏感性均明显高于Chang肝细胞。镉在血管平滑肌细胞中的蓄积比在Chang肝细胞中更明显。暴露于镉后，Chang肝细胞中金属硫蛋白呈浓度依赖性诱导，hSMC中金属硫蛋白含量一直较高，rSMC中金属硫蛋白敏感诱导，A10细胞中金属硫蛋白含量一直较低且在较窄范围内诱导。仅在A10细胞中，还原型谷胱甘肽的细胞内含量更高且被镉显著增强。目前的数据表明，一般而言，血管平滑肌细胞对镉细胞毒性敏感，且无任何物种相关差异，这主要是由于金属在细胞内的蓄积较高。
