Muto N, Dota A, Tanaka T, Itoh N, Okabe M, Inada A, Nakanishi T, Tanaka K
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Biol Pharm Bull. 1995 Nov;18(11):1576-9. doi: 10.1248/bpb.18.1576.
Hinokitiol, a constituent of the wood of Chamaecyparis taiwanensis, was found to induce differentiation of teratocarcinoma F9 cells. When examined by the agar-overlay method, in which expression of plasminogen activator as a differentiation marker protein was detected, this compound exhibited a dose- and time-dependent induction. Induction of differentiation by hinokitiol occurred irreversibly and required its addition for more than 12h. Among its structure-related compounds tested, tropolone and two colchicine-related compounds exerted potent activities comparable to that of hinokitiol. These findings indicate that free tropolone structure in the molecules plays an essential role in inducing differentiation of F9 cells. Hinokitiol showed a strong inhibitory effect of DNA synthesis in very early stages of culture, suggesting that this effect may be responsible for triggering differentiation of F9 cells.
扁柏酚是台湾扁柏木材的一种成分，已发现其可诱导畸胎癌F9细胞分化。采用琼脂覆盖法检测作为分化标记蛋白的纤溶酶原激活剂的表达时，该化合物呈现出剂量和时间依赖性诱导作用。扁柏酚诱导的分化是不可逆的，且需要添加该化合物超过12小时。在测试的与它结构相关的化合物中，托酚酮和两种与秋水仙碱相关的化合物表现出与扁柏酚相当的强活性。这些发现表明分子中的游离托酚酮结构在诱导F9细胞分化中起重要作用。扁柏酚在培养的早期阶段对DNA合成表现出强烈的抑制作用，表明这种作用可能是触发F9细胞分化的原因。
