铁螯合剂诱导胚胎癌F9细胞分化
Induction of differentiation of embryonal carcinoma F9 cells by iron chelators.
作者信息
Tanaka T, Muto N, Itoh N, Dota A, Nishina Y, Inada A, Tanaka K
机构信息
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Res Commun Mol Pathol Pharmacol. 1995 Nov;90(2):211-20.
The effects of several chelators, including hinokitiol, on embryonal carcinoma F9 cell differentiation were assessed by assaying the production of plasminogen activator (PA) as a differentiation marker protein. Hinokitiol and tropolone which were potent differentiation inducers lost their activity following preincubation with Fe2+ and Fe3+ ions. Other metal ions had no or little effect on the hinokitiol-induced differentiation. Of several chelators examined, dithizone induced differentiation as effectively as did hinokitiol and tropolone. Dithizone-induced differentiation was also inhibited by preincubation with Fe3+ ions. It was concluded that some potent iron chelators could trigger the teratocarcinoma F9 cells to differentiate through the chelation with intracellular iron ions.
通过检测纤溶酶原激活剂（PA）作为分化标记蛋白的产生，评估了包括扁柏酚在内的几种螯合剂对胚胎癌细胞F9细胞分化的影响。扁柏酚和托酚酮是有效的分化诱导剂，但在与Fe2+和Fe3+离子预孵育后失去了活性。其他金属离子对扁柏酚诱导的分化没有或几乎没有影响。在所检测的几种螯合剂中，双硫腙诱导分化的效果与扁柏酚和托酚酮一样有效。双硫腙诱导的分化也被Fe3+离子预孵育所抑制。得出的结论是，一些有效的铁螯合剂可以通过与细胞内铁离子螯合来触发畸胎癌F9细胞分化。
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