子宫内膜白细胞亚群的金属蛋白酶分泌

Metalloproteinase secretion by endometrial leukocyte subsets.

作者信息

Shi W, Mognetti B, Campana A, Bischof P

机构信息

Department of Gynaecology and Obstetrics, University of Geneva, Switzerland.

出版信息

Am J Reprod Immunol. 1995 Nov;34(5):299-310. doi: 10.1111/j.1600-0897.1995.tb00956.x.

DOI:10.1111/j.1600-0897.1995.tb00956.x

PMID:8595129

Abstract

PROBLEM

Human endometrium contains up to 40% leukocytes. Since their function is unclear, we cultured these cells separately to analyze their proteolytic potential in vitro.

METHOD

Endometrium was obtained from hysterectomies or from first trimester abortion. After collagenase digestion, the different leukocyte subsets (CD56, CD3, CD19, CD16, CD45) were immuno-separated and cultured. Gelatine zymography and a quantitative type IV gelatinase assay were run on all culture supernatants.

RESULTS

On zymography, all subtypes of endometrial leukocytes released gelatinases. The 72-kDa gelatinase was absent from peripheral leukocyte supernatants, whereas it was secreted by all endometrial leukocytes. The 92-kDa gelatinase was present in all culture supernatants. Large granular lymphocytes (CD56+, LGL) expressed the highest type VI gelatinase activity as compared to all other leukocytes. This activity increased during the cycle in LGL and T cells but remained unchanged in glandular and stromal cells.

CONCLUSIONS

Endometrial leukocytes, particularly LGL, secrete significant amounts of gelatinases.

摘要

问题

人子宫内膜含有高达40%的白细胞。由于其功能尚不清楚，我们将这些细胞单独培养以分析其体外蛋白水解潜能。

方法

子宫内膜取自子宫切除术标本或孕早期流产组织。经胶原酶消化后，对不同的白细胞亚群（CD56、CD3、CD19、CD16、CD45）进行免疫分离并培养。对所有培养上清液进行明胶酶谱分析和IV型明胶酶定量测定。

结果

在酶谱分析中，子宫内膜白细胞的所有亚型均释放明胶酶。外周血白细胞上清液中不存在72 kDa的明胶酶，而所有子宫内膜白细胞均分泌该酶。92 kDa的明胶酶存在于所有培养上清液中。与所有其他白细胞相比，大颗粒淋巴细胞（CD56+，LGL）表达的VI型明胶酶活性最高。LGL和T细胞中的这种活性在月经周期中增加，但在腺细胞和基质细胞中保持不变。