内皮素-1不会使多形核白细胞致敏以增强活性氧代谢产物的产生。

Endothelin-1 does not prime polymorphonuclear leukocytes for enhanced production of reactive oxygen metabolites.

作者信息

Kopprasch S, Gatzweiler A, Kohl M, Schröder H E

机构信息

Technical University of Dresden, Carl Gustav Carus Medical School, Department of Internal Medicine III, Germany.

出版信息

Inflammation. 1995 Dec;19(6):679-87. doi: 10.1007/BF01534571.

DOI:10.1007/BF01534571

PMID:8595934

Abstract

UNLABELLED

The in vitro effect of endothelin-1 (ET-1) on the capacity of polymorphonuclear leukocytes (PMNLs) to generate reactive oxygen species (ROS) was investigated. Human PMNLs were separated from healthy volunteers and preincubated for 10 min. at 37 degrees C with varying concentrations (10(-7)-10(-12) M) of ET-1. After subsequent stimulation with FMLP (10(-7) M) or opsonized zymosan (0.5 mg/ml) the intra- and extracellular generation of ROS was assessed by luminol-amplified chemiluminescence, superoxide radical (.O2-) and hydrogen peroxide (H2O2) production.

RESULTS

ET-1 alone failed to stimulate ROS generation. Neither the capacity for extracellular generation of oxygen metabolites nor the production of ROS with an intracellular origin was changed after preincubation of PMNLs with ET-1. ET-1 did not cause a shift of the .O2-/H2O2 production ratio after stimulation of PMNLs with FMLP. These findings suggest that ET-1 in vitro does not prime human PMNLs for enhanced production of ROS.

摘要

未标记

研究了内皮素-1（ET-1）对多形核白细胞（PMNLs）产生活性氧（ROS）能力的体外作用。从健康志愿者中分离出人PMNLs，并在37℃下用不同浓度（10^(-7)-10^(-12) M）的ET-1预孵育10分钟。在用FMLP（10^(-7) M）或调理酵母聚糖（0.5 mg/ml）随后刺激后，通过鲁米诺增强化学发光、超氧阴离子（·O2-）和过氧化氢（H2O2）产生来评估ROS的细胞内和细胞外产生。