通过化学发光法测定全血和分离的多形核白细胞中循环吞噬细胞氧化活性的比较。

Comparison of circulating phagocyte oxidative activity measured by chemiluminescence in whole blood and isolated polymorphonuclear leukocytes.

作者信息

Kopprasch S, Graessler J, Kohl M, Bergmann S, Schröder H E

机构信息

Technical University of Dresden, Carl Gustav Carus Medical School, Department of Internal Medicine III, Dresden, Germany.

出版信息

Clin Chim Acta. 1996 Sep 30;253(1-2):145-57. doi: 10.1016/0009-8981(96)06365-6.

DOI:10.1016/0009-8981(96)06365-6

PMID:8879845

Abstract

The generation of reactive oxygen species (ROS) was measured by chemiluminescence (CL) in whole blood and isolated polymorphonuclear leukocytes (PMNLs) in 113 subjects. There were no differences in ROS production in males and females, smokers and non-smokers and no age dependency. A significant correlation was found between whole blood CL parameters and between parameters derived from isolated cells, but there was no correlation when these parameters were compared using isolated PMNLs and whole blood. Variations in serum lipid levels did not account for the variability of CL parameters in blood or PMNLs. Both methodologies had the same validity in demonstrating priming of phagocytes. Zymosan-induced CL was more sensitive in detecting distinct functional states of phagocytes than FMLP-induced CL.

摘要

通过化学发光法（CL）检测了113名受试者全血和分离出的多形核白细胞（PMNLs）中活性氧（ROS）的生成情况。男性与女性、吸烟者与非吸烟者之间的ROS生成没有差异，且不存在年龄依赖性。全血CL参数之间以及从分离细胞得出的参数之间存在显著相关性，但当使用分离的PMNLs和全血比较这些参数时则不存在相关性。血清脂质水平的变化并不能解释血液或PMNLs中CL参数的变异性。两种方法在证明吞噬细胞的启动方面具有相同的有效性。与FMLP诱导的CL相比，酵母聚糖诱导的CL在检测吞噬细胞的不同功能状态时更敏感。