啮齿动物互补组8（ERCC8）对应于科凯恩综合征互补组A。

Rodent complementation group 8 (ERCC8) corresponds to Cockayne syndrome complementation group A.

作者信息

Itoh T, Shiomi T, Shiomi N, Harada Y, Wakasugi M, Matsunaga T, Nikaido O, Friedberg E C, Yamaizumi M

机构信息

Department of Cell Genetics, Kumamoto University School of Medicine, Japan.

出版信息

Mutat Res. 1996 Feb 15;362(2):167-74. doi: 10.1016/0921-8777(95)00046-1.

DOI:10.1016/0921-8777(95)00046-1

PMID:8596535

Abstract

US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

摘要

US31是一种对紫外线敏感的突变细胞系（啮齿动物互补组8），源自小鼠T细胞系L5178Y。我们使用针对这些光产物的单克隆抗体，分析了US31细胞中紫外线诱导的环丁烷嘧啶二聚体和（6-4）光产物的去除动力学。虽然几乎所有的（6-4）光产物在紫外线照射后6小时内都得到了修复，但即使在紫外线照射后24小时，仍有超过70%的环丁烷嘧啶二聚体未被修复。这些动力学与科凯恩综合征（CS）细胞的相似。由于US31细胞的细胞融合和转染效率较低，这阻碍了互补试验和基因克隆，我们通过将US31细胞与经X射线照射的正常小鼠成纤维细胞LTA细胞融合，构建了成纤维细胞互补组8细胞系（6L1030）。使用6L1030细胞进行细胞融合和转染的互补试验表明，啮齿动物互补组8对应于CS互补组A。