Eriksen L, Nielsen K
Nord Vet Med. 1977 Mar;29(3):144-50.
Blood eosinophils were determined in eight 7--13-month-old calves that had been reared under parasite-free conditions. Eosinophil counts were obtained by three methods, a) a direct count, b) a differential count and c) an indirect count (i.e. differential count x total leucocyte count). Methodological errors of these methods were determined. Upper limits of 1,000 eosinophils/micronl (direct count) and 9.5 per cent eosinophils (differential count) have been estimated an suitable working standards for calves of this age group. The distribution of eosinophils was skew, most direct counts being below 700 cells/micronl and most differential counts being below 7.5 per cent. A high degree of correlation was found between results obtained by either method, but an even higher correlation was found between direct and indirect counts.
在无寄生虫条件下饲养的8头7至13月龄小牛中测定了血液嗜酸性粒细胞。嗜酸性粒细胞计数通过三种方法获得:a)直接计数,b)分类计数,c)间接计数(即分类计数×白细胞总数)。确定了这些方法的方法学误差。估计1000个嗜酸性粒细胞/微升(直接计数)和9.5%嗜酸性粒细胞(分类计数)的上限是该年龄组小牛合适的工作标准。嗜酸性粒细胞的分布呈偏态,大多数直接计数低于700个细胞/微升,大多数分类计数低于7.5%。两种方法得到的结果之间发现高度相关,但直接计数和间接计数之间的相关性更高。