Henkin T M
Department of Microbiology, Ohio State University, Columbus 43210, USA.
FEMS Microbiol Lett. 1996 Jan 1;135(1):9-15. doi: 10.1111/j.1574-6968.1996.tb07959.x.
The CcpA protein has been identified as a key regulator of carbon metabolism in Bacillus subtilis. CcpA is a DNA binding protein in the LacI/GalR transcriptional repressor family, and genes which respond to CcpA contain common cis-acting target sequences (Ccp boxes). A number of pathways involved in carbon source utilization are repressed by CcpA, while at least one gene which is involved in excretion of excess carbon is activated by CcpA. Genes repressed by CcpA generally contain Ccp boxes within or downstream of the promoter, while ackA, which is activated by CcpA, contains Ccp boxes upstream of the promoter. It therefore appears that CcpA acts globally to direct carbon flow in B. subtilis.
CcpA蛋白已被确定为枯草芽孢杆菌碳代谢的关键调节因子。CcpA是LacI/GalR转录抑制因子家族中的一种DNA结合蛋白，响应CcpA的基因含有共同的顺式作用靶序列（Ccp框）。许多参与碳源利用的途径受到CcpA的抑制，而至少一个参与过量碳排泄的基因被CcpA激活。被CcpA抑制的基因通常在启动子内部或下游含有Ccp框，而被CcpA激活的ackA基因在启动子上游含有Ccp框。因此，CcpA似乎在全局上指导枯草芽孢杆菌中的碳流。
