Sacchi C T, Tondella M L, Gorla M C, de Lemos P S, Melles C E, de Paiva M V, Rodrigues D S, Andrade A J, Ribeiro M O, Sperb A
Bacteriology Division, Adolfo Lutz Institute, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 1995 Jul-Aug;37(4):281-9. doi: 10.1590/s0036-46651995000400001.
In the present study we report the results of an analysis, based on serotyping, multilocus enzyme electrophoresis (MEE), and ribotyping of N. meningitidis serogroup C strains isolated from patients with meningococcal disease (MD) in Rio Grande do Sul (RS) and Santa Catarina (SC) States, Brazil, as the Center of Epidemiology Control of Ministry of Health detected an increasing of MD cases due to this serogroup in the last two years (1992-1993). We have demonstrated that the MD due to N.meningitidis serogroup C strains in RS and SC States occurring in the last 4 years were caused mainly by one clone of strains (ET 40), with isolates indistinguishable by serogroup, serotype, subtype and even by ribotyping. One small number of cases that were not due to an ET 40 strains, represent closely related clones that probably are new lineages generated from the ET 40 clone referred as ET 11A complex. We have also analyzed N.meningitidis serogroup C strains isolated in the greater São Paulo in 1976 as representative of the first post epidemic year in that region. The ribotyping method, as well as MEE, could provide useful information about the clonal characteristics of those isolates and also of strains isolated in south Brazil. The strains from 1976 have more similarity with the actual endemic than epidemic strains, by the ribotyping, sulfonamide sensitivity, and MEE results. In conclusion, serotyping with monoclonal antibodies (C:2b:P1.3), MEE (ET 11 and ET 11A complex), and ribotyping by using ClaI restriction enzyme (Rb2), were useful to characterize these epidemic strains of N.meningitidis related to the increased incidence of MD in different States of south Brazil. It is mostly probable that these N.meningitidis serogroup C strains have poor or no genetic correlation with 1971-1975 epidemic serogroup C strains. The genetic similarity of members of the ET 11 and ET 11A complex were confirmed by the ribotyping method by using three restriction endonucleases.
在本研究中，我们报告了一项分析结果，该分析基于对从巴西南里奥格兰德州（RS）和圣卡塔琳娜州（SC）的脑膜炎球菌病（MD）患者中分离出的C群脑膜炎奈瑟菌菌株进行血清分型、多位点酶电泳（MEE）和核糖分型。因为巴西卫生部流行病学控制中心发现，在过去两年（1992 - 1993年）中，由该血清群引起的MD病例有所增加。我们已经证明，过去4年在RS和SC州发生的由C群脑膜炎奈瑟菌菌株引起的MD主要由一个菌株克隆（ET 40）导致，其分离株在血清群、血清型、亚型甚至核糖分型方面都无法区分。少数非ET 40菌株引起的病例代表密切相关的克隆，可能是从被称为ET 11A复合体的ET 40克隆产生的新谱系。我们还分析了1976年在大圣保罗分离出的C群脑膜炎奈瑟菌菌株，作为该地区首个流行后年份的代表。核糖分型方法以及MEE能够提供有关这些分离株以及在巴西南部分离出的菌株的克隆特征的有用信息。通过核糖分型、磺胺敏感性和MEE结果，1976年的菌株与当前的地方流行菌株比流行菌株有更多相似性。总之，使用单克隆抗体（C:2b:P1.3）进行血清分型、MEE（ET 11和ET 11A复合体）以及使用ClaI限制酶进行核糖分型（Rb2），有助于鉴定与巴西南部不同州MD发病率增加相关的这些C群脑膜炎奈瑟菌流行菌株。很可能这些C群脑膜炎奈瑟菌菌株与1971 - 1975年流行的C群菌株遗传相关性较差或无相关性。通过使用三种限制内切酶的核糖分型方法证实了ET 11和ET 11A复合体成员的遗传相似性。
