Cho M Y, Jung S H, Kim T S
Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, Seoul, Korea.
Yonsei Med J. 1995 Dec;36(6):521-6. doi: 10.3349/ymj.1995.36.6.521.
Abnormalities of the p53 gene are the most common molecular change in human cancer. In the central nervous system, mutant p53 gene is frequently identified in the tumors with astrocytic differentiation. To investigate the relation between histologic subtypes and p53 protein overexpression, we examined 81 cases of astrocytic neoplasms (24 benign astrocytoma, 28 anaplastic astrocytoma and 29 glioblastoma multiforme) using the standard immunohistochemical method. All were formalin-fixed and paraffin-embedded tissue. The p53 immunoreactivity was found in 4/24 benign astrocytoma, 18/28 anaplastic astrocytoma, 22/29 glioblastoma multiforme. The degree of immunoreactivity closely correlated with histologic subtypes (p < 0.001). Overall p53 protein expression was most frequently detected in glioblastoma multiforme, but strong immunoreactivity (3+) was more frequently found in the anaplastic astrocytoma than in glioblastoma multiforme. Although the frequency of p53 protein expression is low, 4 benign astrocytoma showed distinct nuclear staining. In conclusion the malignant progression of astrocytic neoplasms may be associated with increasing expression of p53 protein.
p53基因异常是人类癌症中最常见的分子改变。在中枢神经系统中，突变型p53基因在具有星形细胞分化的肿瘤中经常被发现。为了研究组织学亚型与p53蛋白过表达之间的关系，我们使用标准免疫组织化学方法检查了81例星形细胞瘤（24例良性星形细胞瘤、28例间变性星形细胞瘤和29例多形性胶质母细胞瘤）。所有样本均为福尔马林固定、石蜡包埋组织。在24例良性星形细胞瘤中有4例、28例间变性星形细胞瘤中有18例、29例多形性胶质母细胞瘤中有22例发现p53免疫反应性。免疫反应程度与组织学亚型密切相关（p<0.001）。总体而言，p53蛋白表达最常在多形性胶质母细胞瘤中检测到，但间变性星形细胞瘤中强免疫反应性（3+）的发生率高于多形性胶质母细胞瘤。虽然p53蛋白表达频率较低，但4例良性星形细胞瘤显示出明显的核染色。总之，星形细胞瘤的恶性进展可能与p53蛋白表达增加有关。
