Howng S L, Hwang S L, Chai C Y, Lin H J
Division of Neurosurgery, Kaohsiung Medical College Hospital, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1996 May;12(5):279-84.
P53 immunohistochemistry in astrocytic tumors has usually been evaluated by the percentage of positive cells. However, in this study we analyzed the P53 immunopositive cells by their patterns of distribution. Formalin-fixed and paraffin-embedded sections from 38 patients with astrocytic tumors were examined. The distribution pattern of P53 immunostaining cells was divided into 3 types: negative, locally scattered, and diffuse clustering. There were 2 positive stains in 5 astrocytomas (40%), 12 positive in 24 anaplastic astrocytomas (50%), and 7 positive in 9 glioblastoma multiformes (78%). In astrocytomas, the positive cells were locally scattered. In anaplastic astrocytoma and GBM, the positive cells appeared locally scattered or as diffuse clustering. For the variant immunoreactive expression, the mean ages for patients with negative, locally scattered and diffusely clustered P53 immunostaining were as follows: 51.4, 52.6, and 28.4 years (P < 0.01), respectively. In anaplastic astrocytoma and GBM, the diffusely clustered pattern was more common in younger patients, whereas elderly patients in same groups tended to have few or no P53 immunopositive cells. Thus, our results implicate that clonal expansion of P53 immunopositive cells is associated with brain tumor progression.
星形细胞瘤中的P53免疫组化通常通过阳性细胞百分比进行评估。然而，在本研究中，我们根据P53免疫阳性细胞的分布模式对其进行了分析。对38例星形细胞瘤患者的福尔马林固定石蜡包埋切片进行了检查。P53免疫染色细胞的分布模式分为3种类型：阴性、局灶性散在和弥漫性聚集。5例星形细胞瘤中有2例呈阳性染色（40%），24例间变性星形细胞瘤中有12例呈阳性（50%），9例多形性胶质母细胞瘤中有7例呈阳性（78%）。在星形细胞瘤中，阳性细胞局灶性散在分布。在间变性星形细胞瘤和胶质母细胞瘤中，阳性细胞呈局灶性散在或弥漫性聚集分布。对于不同的免疫反应性表达，P53免疫染色呈阴性、局灶性散在和弥漫性聚集的患者的平均年龄分别为：51.4岁、52.6岁和28.4岁（P<0.01）。在间变性星形细胞瘤和胶质母细胞瘤中，弥漫性聚集模式在年轻患者中更为常见，而同一组中的老年患者P53免疫阳性细胞较少或无。因此，我们的结果表明，P53免疫阳性细胞的克隆性扩增与脑肿瘤进展相关。
