Peris K, Caracciolo E, Weirich G, Chimenti S
Department of Dermatology, University of L'Aquila, Italy.
Am J Dermatopathol. 1995 Oct;17(5):506-10. doi: 10.1097/00000372-199510000-00013.
A 60-year-old woman was examined for erythematous plaques and nodules on the back that had appeared 1 year earlier. Histologic examination of a skin biopsy specimen showed a dense, diffuse infiltrate throughout the dermis and subcutis, composed mainly of centroblasts and centrocytes. Immunohistochemistry confirmed positivity of the neoplastic cells for L26 (CD20) and LN1 (CDw75) antibodies and negativity for UCHL1 (CD45RO), polyclonal anti-CD3, anti-kappa, and anti-lambda antibodies. Polymerase chain reaction (PCR) analysis on paraffin-embedded tissue sections of the cutaneous lesion showed immunoglobulin heavy-chain (VDJ) and kappa-chain gene rearrangement. Routine laboratory studies were within normal limits. The staging workup of the patient (bone marrow biopsy, computed tomographic scans of the chest and abdomen) showed no abnormalities. Based on clinicopathologic findings and molecular analysis, a diagnosis of primary cutaneous centroblastic/centrocytic lymphoma was made. The patient was treated with intra- and perilesionally administered recombinant interferon alpha-2a (3 x 10(6) IU) three times a week for 2 months. Complete response was achieved, and no evidence of recurrence has been observed after 18 months of follow-up.
一名60岁女性因背部出现红斑性斑块和结节前来就诊，这些症状于1年前出现。皮肤活检标本的组织学检查显示，整个真皮层和皮下组织有密集、弥漫性浸润，主要由中心母细胞和中心细胞组成。免疫组织化学证实肿瘤细胞对L26（CD20）和LN1（CDw75）抗体呈阳性，而对UCHL1（CD45RO）、多克隆抗CD3、抗κ和抗λ抗体呈阴性。对皮肤病变石蜡包埋组织切片进行聚合酶链反应（PCR）分析，显示免疫球蛋白重链（VDJ）和κ链基因重排。常规实验室检查结果均在正常范围内。患者的分期检查（骨髓活检、胸部和腹部计算机断层扫描）未发现异常。根据临床病理表现和分子分析，诊断为原发性皮肤中心母细胞/中心细胞淋巴瘤。患者接受了每周3次、每次在皮损内及皮损周围注射重组干扰素α-2a（3×10⁶IU），共治疗2个月。治疗后达到完全缓解，随访18个月未观察到复发迹象。
