[Measurement of water content and distribution using whole-body multifrequency impedance in a healthy population in diseases of the kidney].

BACKGROUND

The whole-body multifrequency impedometry (MFI) is a new noninvasive method suggested for assessment of hydration status and distribution of the total body water into extra- and intracellular compartment. However, due to its as yet limited use reference values are still lacking. The aim of this work was to evaluate total body water (TBW) and its intra- (VIC) and extracellular compartment (VEC) from electrical resistances of intra- and extracellular fluid and to measure cell membrane capacity by means of MFI in healthy population and in renal patients.

METHODS AND RESULTS

MFI investigation was performed in 21 healthy individuals (group A), 19 patients followed for renal insufficiency but not yet dialysed (group B) and in 15 haemodialysis patients (group C). The bioimpedance analyzer BIS 4000B (Xitron Technologies, San Diego, USA) was used throughout the study. TBW values established by means of MFI corresponded very closely to those ones calculated from Watson's formulae. The ratio VEC/TBW was found to be related to age with regression analysis parameters being very similar both in group A and groups B, C: group A: VEC/TBW = 0.4696 + 0.0011. Age (r = 0.512) groups B, C: VEC/TBW = 0.4574 + 0.0016. Age (r = 0.420). (For group C the post-dialysis values were used). Close correlation of VEC changes with ultrafiltered volume during haemodialysis session (r = 0.87 pri n = 87) indicates mostly extracellular origin of ultrafiltrate. The highest capacity of the cellular wall was found in healthy individuals, the lowest values were seen in the dialysed group. During dialysis the capacity rose to values seen in the non-dialysed group of renal patients. Interindividual scater over 40% in CIC value is caused by differences in body size. Normalized capacity CIC/VIC in healthy group is 0.104 nF/L +/- 10% on average. In renal patients values from 0.73 to 0.9 nF/L were seen. With regard to low variation of CIC/VIC in health, this parameter can be considered a possible candidate for screening assessment of the renal patients' hydration status.

CONCLUSIONS

MFI was found suitable for monitoring of water content and distribution in the body both in out-patient department as well as during haemodialysis.