Selvanayagam P, Rajaraman S
Department of Pathology, University of Texas Medical Branch, Galveston, USA.
Lab Invest. 1996 Mar;74(3):592-9.
A cDNA isolated by a subtractive hybridization procedure detected loss of mtDNA and the mRNA coding for NADH dehydrogenase subunit 3 in 8 of 13 tumor kidney tissues obtained from patients with renal cell carcinoma. Sequencing revealed a stretch of nucleotides homologous to the mitochondrial NADH dehydrogenase subunit 3 gene in the middle of the cDNA. The depletion phenomenon was also observed in five of six renal carcinoma cell lines. In the case of a benign renal oncocytoma, however, the mtDNA content was increased 200% more than that of the adjacent normal tissue. The frequency with which this phenomenon occurs in renal cell carcinomas, but not in other types of cancers, suggests that this may be an important phenotype associated with renal cell neoplastic transformation. However, the absence of any structural alterations within the mitochondrial genome suggests that the depletion may be a secondary event associated with the oncogenic transformation process.
通过消减杂交程序分离出的一种互补DNA（cDNA），在13例肾细胞癌患者的肿瘤肾组织中有8例检测到线粒体DNA（mtDNA）缺失以及编码烟酰胺腺嘌呤二核苷酸（NADH）脱氢酶亚基3的信使核糖核酸（mRNA）缺失。测序显示该cDNA中间有一段与线粒体NADH脱氢酶亚基3基因同源的核苷酸序列。在6个肾癌细胞系中有5个也观察到了这种缺失现象。然而，在良性肾嗜酸细胞瘤中，mtDNA含量比相邻正常组织增加了200%以上。这种现象在肾细胞癌中出现的频率，而在其他类型癌症中未出现，表明这可能是与肾细胞肿瘤转化相关的一种重要表型。然而，线粒体基因组内未发现任何结构改变，提示这种缺失可能是与致癌转化过程相关的继发事件。
