Touati E, Laurent-Winter C, Quillardet P, Hofnung M
Unité de Programmation Moleculaire et de Toxicologie Génetique/CNRS Ura 1444, Institut Pasteur, France.
Mutat Res. 1996 Feb 1;349(2):193-200. doi: 10.1016/0027-5107(95)00178-6.
We have studied the global changes which are induced in exponentially growing cultures of Escherichia coli during treatment with 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000), a very potent mutagen and inducer of the SOS response. A two-dimensional analysis of the proteins synthesized in the presence of this agent was performed. In a strain deficient in SOS induction, the expression of 24 polypeptides was found to be affected by this treatment: 14 corresponded to proteins known to be implicated in different stress responses, particularly chaperones such as DnaK and GroEL. The variation of another protein was detected for the first time in this study: transketolase I, an enzyme of intermediary metabolism which was characterized in the present work by microsequencing. In parallel, the expression of 9 other proteins, still unidentified, was modified by this nitrofuran. They represent potential candidates involved in metabolic and DNA lesions repair specific of R7000 action.
我们研究了在指数生长的大肠杆菌培养物中，用7-甲氧基-2-硝基萘并[2,1-b]呋喃（R7000）处理时所引发的全局变化。R7000是一种非常有效的诱变剂和SOS反应诱导剂。我们对在该试剂存在下合成的蛋白质进行了二维分析。在一个缺乏SOS诱导的菌株中，发现24种多肽的表达受此处理影响：其中14种对应于已知参与不同应激反应的蛋白质，特别是伴侣蛋白，如DnaK和GroEL。在本研究中首次检测到另一种蛋白质的变化：转酮醇酶I，一种中间代谢酶，在本研究中通过微量测序对其进行了表征。同时，9种其他尚未鉴定的蛋白质的表达也因这种硝基呋喃而发生改变。它们代表了参与R7000作用特异性的代谢和DNA损伤修复的潜在候选蛋白。
