Krin Evelyne, Laurent-Winter Christine, Bertin Philippe N, Danchin Antoine, Kolb Annie
Unité de Génétique des Génomes Bactériens, Institut Pasteur, 75724 Paris Cedex 15, France.
J Bacteriol. 2003 May;185(10):3139-46. doi: 10.1128/JB.185.10.3139-3146.2003.
The cAMP-catabolite activator protein (CAP) complex is a pleiotropic regulator that regulates a vast number of Escherichia coli genes, including those involved in carbon metabolism. We identified two new targets of this complex: argG, which encodes the arginosuccinate synthase involved in the arginine biosynthetic pathway, and metY, which encodes one of the two methionine tRNA initiators, tRNAf2Met. The cAMP-CAP complex activates argG transcription and inhibits metY transcription from the same DNA position. We also show that ArgR, the specific repressor of the arginine biosynthetic pathway, together with its arginine cofactor, acts on the regulation of metY mediated by CAP. The regulation of the two divergent promoters is thus simultaneously controlled not only by the cAMP-CAP complex, a global regulator, but also by a specific regulator of arginine metabolism, suggesting a previously unsuspected link between carbon metabolism and translation initiation.
环磷酸腺苷 - 分解代谢物激活蛋白（CAP）复合物是一种多效调节因子，可调节大量大肠杆菌基因，包括参与碳代谢的基因。我们鉴定出该复合物的两个新靶点：argG，其编码参与精氨酸生物合成途径的精氨琥珀酸合酶；以及metY，其编码两种甲硫氨酸tRNA起始物之一，即tRNAf2Met。cAMP - CAP复合物在同一DNA位置激活argG转录并抑制metY转录。我们还表明，精氨酸生物合成途径的特异性阻遏物ArgR与其精氨酸辅因子一起，作用于由CAP介导的metY调节。因此，两个 divergent 启动子的调节不仅同时受到全局调节因子cAMP - CAP复合物的控制，还受到精氨酸代谢特异性调节因子的控制，这表明碳代谢与翻译起始之间存在先前未被怀疑的联系。
