P9.1 - P9.2外周延伸有助于引导嗜热四膜虫核酶的折叠。
The P9.1-P9.2 peripheral extension helps guide folding of the Tetrahymena ribozyme.
作者信息
Zarrinkar P P, Williamson J R
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
Nucleic Acids Res. 1996 Mar 1;24(5):854-8. doi: 10.1093/nar/24.5.854.
Abstract
We have previously proposed a hierarchical model for the folding mechanism of the Tetrahymena ribozyme that may illustrate general features of the folding pathways of large RNAs. While the role of elements in the conserved catalytic core of this ribozyme during the folding process is beginning to emerge, the participation of non-conserved peripheral extensions in the kinetic folding mechanism has not yet been addressed. We now show that the 3'-terminal P9.1-P9.2 extension of the Tetrahymena ribozyme plays an important role during the folding process and appears to guide formation of the catalytic core.
摘要
我们之前提出了一种针对嗜热四膜虫核酶折叠机制的分层模型，该模型可能阐释了大型RNA折叠途径的一般特征。虽然在折叠过程中，该核酶保守催化核心中的元件作用已开始显现，但非保守外周延伸在动力学折叠机制中的参与情况尚未得到探讨。我们现在表明，嗜热四膜虫核酶的3'端P9.1 - P9.2延伸在折叠过程中发挥重要作用，并且似乎引导催化核心的形成。
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