抗ras核酶的组织特异性表达抑制人恶性黑色素瘤细胞的增殖。
Tissue-specific expression of an anti-ras ribozyme inhibits proliferation of human malignant melanoma cells.
作者信息
Ohta Y, Kijima H, Ohkawa T, Kashani-Sabet M, Scanlon K J
机构信息
Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA 91010, USA.
出版信息
Nucleic Acids Res. 1996 Mar 1;24(5):938-42. doi: 10.1093/nar/24.5.938.
Abstract
In this study, we have compared the efficacy of a tissue-specific promoter (tyrosinase promoter) with a viral promoter to express anti-ras ribozyme RNA in human melanoma cells. The retroviral vector containing the tyrosinase promoter was superior in its ability to suppress the human melanoma phenotype in vitro as characterized by changes in growth, melanin synthesis, morphology and H-ras gene expression. These data support the use of tissue-specific expression of anti-oncogene ribozymes as a rational therapeutic strategy in human cancers.
摘要
在本研究中，我们比较了组织特异性启动子（酪氨酸酶启动子）与病毒启动子在人黑素瘤细胞中表达抗ras核酶RNA的效果。含有酪氨酸酶启动子的逆转录病毒载体在体外抑制人黑素瘤表型的能力方面表现更优，其表现为生长、黑色素合成、形态及H-ras基因表达的变化。这些数据支持将抗癌基因核酶的组织特异性表达作为人类癌症的一种合理治疗策略。
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本文引用的文献
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3
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In Vivo. 1993 Nov-Dec;7(6A):471-6.
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Ras mutations in human melanoma: a marker of malignant progression.人类黑色素瘤中的Ras突变：恶性进展的一个标志物。
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